Anti-H6PD Antibody (CAB6440)

Antibody Name:	Anti-H6PD Antibody
Antibody SKU:	CAB6440
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QELQ GHVS IILL GATG DLAK KYLW QGLF QLYL DEAG RGHS FSFH GAAL TAPK QGQE LMAK ALES LSCP KDMA PSHC AEHK DQFL QLSQ YRQL KTAE DYQA LNKD IEAQ LQHA GLRE AGRI FYFS VPPF AYED IARN INSS CRPG PGAW LRVV LEKP FGHD HFSA QQLA TELG TFFQ EEEM Y
Gene ID:	9563
Uniprot:	O95479
Cellular Location:	Endoplasmic reticulum lumen
Calculated MW:	88kDa
Observed MW:	89kDa

Synonyms:	H6PD, CORTRD1, G6PDH, GDH
Background:	There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.

UniProt Protein Function:	H6PD: Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
UniProt Protein Details:	Protein type:Oxidoreductase; EC 3.1.1.31; EC 1.1.1.47; Carbohydrate Metabolism - pentose phosphate pathway; Hydrolase Chromosomal Location of Human Ortholog: 1p36 Disease: Cortisone Reductase Deficiency 1
NCBI Summary:	There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq, Jul 2008]
UniProt Code:	O95479
NCBI GenInfo Identifier:	108884809
NCBI Gene ID:	9563
NCBI Accession:	O95479.2
UniProt Secondary Accession:	O95479,Q4TT33, Q66I35, Q68DT3,
UniProt Related Accession:	O95479
Molecular Weight:	88,893 Da
NCBI Full Name:	GDH/6PGL endoplasmic bifunctional protein
NCBI Synonym Full Names:	hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase
NCBI Official Symbol:	H6PD
NCBI Official Synonym Symbols:	GDH; G6PDH; CORTRD1
NCBI Protein Information:	GDH/6PGL endoplasmic bifunctional protein
UniProt Protein Name:	GDH/6PGL endoplasmic bifunctional protein
UniProt Synonym Protein Names:	Glucose 1-dehydrogenase (EC:1.1.1.47)Alternative name(s):Hexose-6-phosphate dehydrogenase
Protein Family:	GDH/6PGL endoplasmic bifunctional protein
UniProt Gene Name:	H6PD
UniProt Entry Name:	G6PE_HUMAN

	Western blot analysis of extracts of various cell lines, using H6PD Antibody (CAB6440) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using H6PD antibody (CAB6440) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using H6PD antibody (CAB6440). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
H6PD Antibody (PACO09663)	Human Glutamate dehydrogenase / GDH ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)