The Hamster TGF Beta (Transforming Growth Factor Beta) ELISA Kit is specifically designed for the accurate and reliable detection of TGF Beta levels in hamster serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications. Transforming Growth Factor Beta is a key signaling molecule involved in regulating cell growth, differentiation, and immune response. It plays a crucial role in various biological processes, including tissue regeneration, wound healing, and immune modulation.
Monitoring TGF Beta levels can provide valuable insights into the pathophysiology of different diseases and aid in the development of targeted therapies. Overall, the Hamster TGF Beta ELISA Kit is an essential tool for researchers studying TGF Beta-related mechanisms and exploring its role in various diseases and conditions. Its high performance and user-friendly design make it a valuable asset for laboratories conducting in-depth research in the field of cell biology and immunology.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-103%
83-100%
82-94%
EDTA Plasma (n = 5)
86-97%
83-89%
86-98%
Heparin Plasma (n = 5)
88-101%
87-99%
90-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-101
95
EDTA Plasma (n = 5)
89-104
96
Heparin Plasma (n = 5)
89-97
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
