Anti-HAND2 Antibody (CAB7044)

Antibody Name:	Anti-HAND2 Antibody
Antibody SKU:	CAB7044
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human HAND2 (NP_068808.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human HAND2 (NP_068808.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLV GGFP HHPV VHHE GYPF AAAA AAAA AAAA SRCS HEEN PYFH GWLI GHPE MSPP DYSM ALSY SPEY ASGA AGLD HSHY GGVP PGAG PPGL GGPR PVKR RGTA NRKE RRRT QSIN SAFA ELRE CIPN VPAD TKLS KIKT LRLA TSYI AYLM DLLA KDDQ NGEA EAFK AEIK KTDV KEEK RKKE LNEI LKST VSSN DKKT KGRT GWPQ HVWA LELK Q
Gene ID:	9464
Uniprot:	P61296
Cellular Location:	Nucleus
Calculated MW:	20kDa/23kDa
Observed MW:	26kDa

Synonyms:

HAND2, DHAND2, Hed, Thing2, bHLHa26, dHand

Background:

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.

UniProt Protein Function:	HAND2: Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 4q33 Cellular Component: nuclear chromatin; protein complex; transcription factor complex Molecular Function:AT DNA binding; protein binding; protein homodimerization activity; transcription activator binding; transcription coactivator activity; transcription factor binding Biological Process: adult heart development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; thymus development
NCBI Summary:	The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq, Jul 2008]
UniProt Code:	P61296
NCBI GenInfo Identifier:	47117699
NCBI Gene ID:	9464
NCBI Accession:	P61296.1
UniProt Secondary Accession:	P61296,O95300, O95301, P97833, Q494T1, B6ECG9,
UniProt Related Accession:	P61296
Molecular Weight:	20,269 Da
NCBI Full Name:	Heart- and neural crest derivatives-expressed protein 2
NCBI Synonym Full Names:	heart and neural crest derivatives expressed 2
NCBI Official Symbol:	HAND2
NCBI Official Synonym Symbols:	Hed; dHand; DHAND2; Thing2; bHLHa26
NCBI Protein Information:	heart- and neural crest derivatives-expressed protein 2
UniProt Protein Name:	Heart- and neural crest derivatives-expressed protein 2
UniProt Synonym Protein Names:	Class A basic helix-loop-helix protein 26; bHLHa26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2; dHAND
Protein Family:	Heart- and neural crest derivatives-expressed protein
UniProt Gene Name:	HAND2
UniProt Entry Name:	HAND2_HUMAN

	Western blot analysis of extracts of various cell lines, using HAND2 antibody (CAB7044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using HAND2 antibody (CAB7044). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)