The HBA1 Polyclonal Antibody (CAB7322) is a valuable tool for researchers studying hemoglobin subunit alpha 1 (HBA1) and its role in oxygen transport and delivery in the body. This antibody, produced in rabbits, has high specificity and sensitivity for detecting HBA1 in human samples, making it ideal for use in Western blot applications.HBA1 is a critical component of hemoglobin, the protein responsible for binding and transporting oxygen from the lungs to the tissues. Dysregulation of HBA1 expression or function can lead to various disorders, including anemia and oxygen transport deficiencies. By using the HBA1 Polyclonal Antibody, researchers can study the expression levels and localization of HBA1 in different cell types and tissues, providing insights into its physiological and pathological roles.
Furthermore, the HBA1 Polyclonal Antibody can be utilized in studies investigating the impact of genetic mutations or environmental factors on HBA1 function, paving the way for potential therapeutic interventions targeting hemoglobin-related disorders. With its versatility and reliability, this antibody is a valuable asset for research in the fields of hematology, biochemistry, and molecular biology.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Hemoglobin subunit alpha (Hemoglobin subunit alpha (HBA1)) (NP_000508.1).
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Purification Method:
Affinity purification
Gene ID:
3039
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Hemoglobin subunit alpha (Hemoglobin subunit alpha (HBA1)) antibody (CAB7322) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
