Western blot analysis of various lysates, using HCoV-229E Spike S1 antibody (CAB22605) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The HCoV-229E Spike S1 Monoclonal Antibody (CAB22605) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed for use in various immunological applications, is highly specific and reactive with human samples.The HCoV-229E Spike S1 Monoclonal Antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. By targeting the Spike protein of the HCoV-229E virus, this antibody enables researchers to study the virus's structure, function, and interactions with host cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against HCoV-229E samples.
Product Name:
HCoV-229E Spike S1 Monoclonal Antibody
SKU:
CAB22605
Size:
20μL, 100μL
Reactivity:
HCoV-229E
Clone Number:
ARC57845
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Recombinant Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag)
Calculated MW:
129kDa
Observed MW:
160kDa(RecombinantProtein)
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method
Affinity purification
Gene ID
918758
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using HCoV-229E Spike S1 Rabbit mAb (CAB22605) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Negative control (NC): 293T. Exposure time: 180s.
