Western blot analysis of Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag), using HCoV-229E Spike S2 antibody (CAB22604) at 1:70000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The HCoV-229E Spike S2 Monoclonal Antibody (CAB22604) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal techniques, is highly specific and reactive with human samples, making it an ideal tool for studying the mechanisms of infection and immune response against this coronavirus strain.The Spike S2 protein of HCoV-229E is a critical component involved in viral entry into host cells and plays a key role in the infectivity and pathogenesis of the virus. By targeting this protein with the monoclonal antibody, researchers can elucidate the molecular interactions between the virus and host cells, paving the way for the development of novel therapeutics and vaccines against HCoV-229E.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against HCoV-229E samples.
Product Name:
HCoV-229E Spike S2 Monoclonal Antibody
SKU:
CAB22604
Size:
20μL, 100μL
Reactivity:
HCoV-229E
Clone Number:
ARC57280
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:3000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag)
Calculated MW:
129kDa
Observed MW:
160kDa
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method
Affinity purification
Gene ID
918758
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag) using HCoV-229E Spike S2 Rabbit mAb (CAB22604) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 100ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
