Histamine Assay Kit
HISTAMINE (C5H9N3) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant that mostly found in the Scombridae family fishes such as tuna and mackerel or other seafood products when improperly handled or stored. The consumption of foods containing high level of histamine may lead to an allergy-like food poisoning known as scombroid poisoning.Assay Genie's histamine assay kit is based on histamine dehydrogenase catalyzed oxidation of histamine in which the formed electron mediator reduces a formazan (MTT) reagent. The intensity of product color, measured at 565 nm is directly proportional to histamine concentration in the sample.
ApplicationsFor quantitative determination of histamine in food, beverage and agricultural products.
- Fast and sensitive. Use of 20 uL sample. Linear detection range 0.0025 - 1 mM (0.3 - 111 ppm) histamine in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Histamine Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL Standard: 1.0 mL 20 mM Histamine HDH Enzyme: 120 uL|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate or cuvette reader.|
|Method of Detection:||OD565nm|
|Detection Limit:||2.5 uM|
|Samples:||Food, beverage and agricultural products|
|Protocol Length:||30 min|
|Storage:||Store all components at -20°C upon receiving|
|Shelf Life:||6 months|