The His (Histamine) ELISA Kit is specifically designed for the precise measurement of histamine levels in biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for research in various fields.Histamine is a biogenic amine that plays a crucial role in immune response, inflammatory processes, and neurotransmission. Dysregulation of histamine levels has been associated with allergies, asthma, and other inflammatory disorders, making it a key biomarker for studying these conditions and developing new treatment strategies. With the His (Histamine) ELISA Kit, researchers can confidently measure histamine levels in biological samples, leading to a better understanding of histamine's role in health and disease. Upgrade your research with this reliable and efficient assay tool.
Product Name:
HIS (Histamine) ELISA Kit
SKU:
UNES00022
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
