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Histone H3 Rabbit Polyclonal Antibody (CAB2348)

SKU:
CAB2348
Product Type:
Antibody
Applications:
WB
IHC
IF
IP
ChIP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Histone H3 Antibody CAB2348
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Histone H3 Antibody CAB2348
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Histone H3 Antibody CAB2348
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Histone H3 Antibody CAB2348
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-Histone H3 Antibody CAB2348
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Histone H3 Rabbit Polyclonal Antibody
SKU:CAB2348
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3 (NP_003520.1).
Sequence:VKKP HRYR PGTV ALRE IRRY QKST ELLI RKLP FQRL VREI AQDF KTDL RFQS SAVM ALQE ACEA YLVG LFED TNLC AIHA KRVT IMPK DIQL ARRI RGER A
Tested Applications:WB IHC-P IF/ICC IP ChIP ELISA
Recommended Dilution:WB,1:2000 - 1:10000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 5μg-10μg of Chromatin
Synonyms:H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3
Positive Sample:293F,NIH/3T3,PC-12
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:16kDa
Observed MW:17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Purification Method:Affinity purification
Gene ID:8290/8350
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Histone H3 Rabbit pAb (CAB2348) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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