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Histone H4 Rabbit Polyclonal Antibody (CAB1131)

SKU:
CAB1131
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 2 Anti-Histone H4 Antibody CAB1131
  • Cell Biology Antibodies 2 Anti-Histone H4 Antibody CAB1131
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Histone H4 Rabbit Polyclonal Antibody
SKU:CAB1131
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_003529.1).
Sequence:MSGR GKGG KGLG KGGA KRHR KVLR DNIQ GITK PAIR RLAR RGGV KRIS GLIY EETR GVLK VFLE NVIR DAVT YTEH AKRK TVTA MDVV YALK RQGR TLYG
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4A; Histone H4
Positive Sample:HeLa,HepG2,Raji,MCF-7,NIH3T3,Mouse Thymus
Conjugate:Unconjugated
Cellular Localization:extracellular exosome, extracellular region, nucleoplasm, nucleus
Calculated MW:11kDa
Observed MW:11kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Purification Method:Affinity purification
Gene ID:8359
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using Histone H4 Rabbit pAb (CAB1131) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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