Anti-HLA-DQA1 Antibody (CAB2168)

Antibody Name:	Anti-HLA-DQA1 Antibody
Antibody SKU:	CAB2168
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-216 of human HLA-DQA1 (NP_002113.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, HT-29, Lovo, Mouse brain, Mouse stomach

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-216 of human HLA-DQA1 (NP_002113.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EDIV ADHV ASCG VNLY QFYG PSGQ YTHE FDGD EQFY VDLE RKET AWRW PEFS KFGG FDPQ GALR NMAV AKHN LNIM IKRY NSTA ATNE VPEV TVFS KSPV TLGQ PNTL ICLV DNIF PPVV NITW LSNG QSVT EGVS ETSF LSKS DHSF FKIS YLTF LPSA DEIY DCKV EHWG LDQP LLKH WEPE IPAP MSEL T
Gene ID:	3117
Uniprot:	P01909
Cellular Location:	Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane
Calculated MW:	27kDa
Observed MW:	28kDa-37KD

Synonyms:

HLA-DQA1, CELIAC1, DQ-A1, HLA-DQA, CD, GSE, class II

Background:

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.

UniProt Protein Function:	HLA-DQA1 iso4:
UniProt Protein Details:	Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex; external side of plasma membrane Molecular Function:MHC class II receptor activity; peptide antigen binding Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; T cell receptor signaling pathway
NCBI Summary:	HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq, Jul 2008]
UniProt Code:	P01909
NCBI GenInfo Identifier:	122188
NCBI Gene ID:	3117
NCBI Accession:	P01909.1
UniProt Related Accession:	P01909
Molecular Weight:
NCBI Full Name:	HLA class II histocompatibility antigen, DQ alpha 1 chain
NCBI Synonym Full Names:	major histocompatibility complex, class II, DQ alpha 1
NCBI Official Symbol:	HLA-DQA1
NCBI Official Synonym Symbols:	DQA1; DQ-A1; CELIAC1; HLA-DQA
NCBI Protein Information:	HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Protein Name:	HLA class II histocompatibility antigen, DQ alpha 1 chain
UniProt Synonym Protein Names:	DC-1 alpha chain; DC-alpha; HLA-DCA; MHC class II DQA1
Protein Family:	HLA class II histocompatibility antigen
UniProt Gene Name:	HLA-DQA1
UniProt Entry Name:	DQA1_HUMAN

	Western blot analysis of extracts of various cell lines, using HLA-DQA1 antibody (CAB2168) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human prostate using HLA-DQA1 antibody (CAB2168) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using HLA-DQA1 antibody (CAB2168) at dilution of 1:100 (40x lens).

Antibodies

Anti-HLA-DQA1 Antibody (CAB11252)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)