null

HLA-DQB1 Rabbit Polyclonal Antibody (CAB20372)

SKU:
CAB20372
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
IDDM1
CELIAC1
HLA-DQB
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:HLA-DQB1 Rabbit Polyclonal Antibody
SKU:CAB20372
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 33-127 of human HLA-DQB1 (NP_002114.3).
Sequence:RDSP EDFV FQFK GMCY FTNG TERV RLVT RYIY NREE YARF DSDV GVYR AVTP QGRP DAEY WNSQ KEVL EGTR AELD TVCR HNYE VAFR GILQ RRV
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:IDDM1; CELIAC1; HLA-DQB; HLA-DQB1
Positive Sample:Raji
Conjugate:Unconjugated
Cellular Localization:plasma membrane
Calculated MW:30kDa
Observed MW:30kDa

HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:3119
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Raji cells, using HLA-DQB1 Rabbit pAb (CAB20372) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Related Products