Anti-HLA-DRB1 Antibody (CAB7685)

Antibody Name:	Anti-HLA-DRB1 Antibody
Antibody SKU:	CAB7685
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human HLA-DRB1 (NP_002115.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, Mouse thymus, Mouse spleen, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human HLA-DRB1 (NP_002115.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GDTR PRFL WQPK RECH FFNG TERV RFLD RYFY NQEE SVRF DSDV GEFR AVTE LGRP DAEY WNSQ KDIL EQAR AAVD TYCR HNYG VVES FTVQ RRVQ PKVT VYPS KTQP LQHH NLLV CSVS GFYP GSIE VRWF LNGQ EEKA GMVS TGLI QNGD WTFQ TLVM LETV PRSG EVYT CQVE HPSV TSPL TVEW RARS ESAQ SKML S
Gene ID:	3123
Uniprot:	P01911/P01912/Q29974
Cellular Location:	Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane
Calculated MW:	30kDa
Observed MW:	30kDa

Synonyms:

HLA-DRB1, DRB1, HLA-DR1B, HLA-DRB, SS1, major histocompatibility complex, class II, DR beta 1

Background:

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.

UniProt Protein Function:	HLA-DRB1 iso2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Genetic variation in HLA-DRB1 is a cause of susceptibility to sarcoidosis type 1 (SS1). Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Belongs to the MHC class II family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cell surface; Golgi membrane; late endosome membrane; lysosomal membrane; membrane; MHC class II protein complex; plasma membrane; trans-Golgi network membrane Molecular Function:peptide antigen binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; polysaccharide assembly with MHC class II protein complex; T cell costimulation; T cell receptor signaling pathway
NCBI Summary:	HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]
UniProt Code:	P01911
NCBI GenInfo Identifier:	166214928
NCBI Gene ID:	3123
NCBI Accession:	P01911.2
UniProt Secondary Accession:	P01911,Q29790, Q29975, Q30142, Q30166, Q32MY7, Q56FN9 Q5Y7B0, Q5Y7B9,
UniProt Related Accession:	P20039,Q5Y7A7,Q95IE3,Q9GIY3,Q29974,P01912,Q30134,P04229,P01911,Q30167
Molecular Weight:	29,966 Da
NCBI Full Name:	HLA class II histocompatibility antigen, DRB1-15 beta chain
NCBI Synonym Full Names:	major histocompatibility complex, class II, DR beta 1
NCBI Official Symbol:	HLA-DRB1
NCBI Official Synonym Symbols:	SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B
NCBI Protein Information:	major histocompatibility complex, class II, DR beta 1
UniProt Protein Name:	HLA class II histocompatibility antigen, DRB1-15 beta chain
UniProt Synonym Protein Names:	DW2.2/DR2.2; MHC class II antigen DRB1*15
Protein Family:	HLA class II histocompatibility antigen
UniProt Gene Name:	HLA-DRB1
UniProt Entry Name:	2B1F_HUMAN

	Western blot analysis of extracts of various cell lines, using HLA-DRB1 antibody (CAB7685) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using HLA-DRB1 antibody (CAB7685). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)