The HLA-DRB1 Antibody (CAB7685) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications.The HLA-DRB1 antibody is validated for use in Western blotting, immunohistochemistry, and flow cytometry, enabling researchers to detect and analyze the expression of HLA-DRB1 in different cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
HLA-DRB1 Antibody
SKU:
CAB7685
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SS1, DRB1, HLA-DRB, HLA-DR1B, HLA-DRB1
Positive Sample:
MCF7, Mouse thymus, Mouse spleen, Mouse heart
Cellular Localization:
Cell Membrane, Endoplasmic Reticulum Membrane, Endosome Membrane, Golgi Apparatus, Late Endosome Membrane, Lysosome Membrane, Single-Pass Type I Membrane Protein, Trans-Golgi Network Membrane.
Calculated MW:
30kDa
Observed MW:
30kDa
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and some alleles have increased frequencies associated with certain diseases or conditions. For example, DRB1*1302 has been related to acute and chronic hepatitis B virus persistence. There are multiple pseudogenes of this gene.
Purification Method
Affinity purification
Gene ID
3123
RRID
AB_2769803
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HLA-DRB1 Rabbit pAb (CAB7685) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (AbGn00020)._Exposure time: 30s.
