Description
HLA-DRA Antibody (CAB1579)
The HLA-DRA Antibody (CAB1579) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in applications such as Western blot and immunohistochemistry.HLA molecules play a crucial role in the immune system, specifically in presenting antigens to T cells to initiate an immune response. The HLA-DRA protein is involved in antigen presentation and is essential for the immune system to recognize and respond to foreign invaders such as viruses and bacteria.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | HLA-DRA Antibody |
| SKU: | CAB1579 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | IKEE HVII QAEF YLNP DQSG EFMF DFDG DEIF HVDM AKKE TVWR LEEF GRFA SFEA QGAL ANIA VDKA NLEI MTKR SNYT PITN VPPE VTVL TNSP VELR EPNV LICF IDKF TPPV VNVT WLRN GKPV TTGV SETV FLPR EDHL FRKF HYLP FLPS TEDV YDCR VEHW GLDE PLLK HWEF DAPS PLPE TTE | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | HLA-DRA1, HLA-DRA |
| Positive Sample: | Raji, Jurkat |
| Cellular Localization: | Cell Membrane, Endoplasmic Reticulum Membrane, Endosome Membrane, Golgi Apparatus, Late Endosome Membrane, Lysosome Membrane, Single-Pass Type I Membrane Protein, Trans-Golgi Network Membrane. |
| Calculated MW: | 29kDa |
| Observed MW: | 37kDa |
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
| Purification Method | Affinity purification |
| Gene ID | 3122 |
| RRID | AB_2763210 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using HLA-DRA Rabbit pAb (CAB1579) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
