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Anti-HLA-E Antibody (CAB14206)

SKU:
CAB14206
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-HLA-E Antibody
Antibody SKU:CAB14206
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-200 of human HLA-E (NP_005507.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, SKOV3, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-200 of human HLA-E (NP_005507.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GSHS LKYF HTSV SRPG RGEP RFIS VGYV DDTQ FVRF DNDA ASPR MVPR APWM EQEG SEYW DRET RSAR DTAQ IFRV NLRT LRGY YNQS EAGS HTLQ WMHG CELG PDGR FLRG YEQF AYDG KDYL TLNE DLRS WTAV DTAA QISE QKSN DASE AEHQ RAYL EDTC VEWL HKYL EKGK ETL
Gene ID:3133
Uniprot:P13747
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:40kDa
Observed MW:50kDa
Synonyms:HLA-6.2, QA1, HLA-E, EA1.2, EA2.1
Background:HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
UniProt Protein Function:HLA-E: Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. Belongs to the MHC class I family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; early endosome membrane; plasma membrane; MHC class I protein complex

Molecular Function:peptide antigen binding; MHC class I protein binding; beta-2-microglobulin binding; receptor binding

Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; positive regulation of natural killer cell mediated immunity; regulation of natural killer cell mediated immunity; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of endogenous peptide antigen via MHC class Ib; protection from natural killer cell mediated cytotoxicity

NCBI Summary:HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
UniProt Code:P13747
NCBI GenInfo Identifier:62912479
NCBI Gene ID:3133
NCBI Accession:NP_005507.3
UniProt Secondary Accession:P13747,Q30169, Q9BT83, Q9GIY7, Q9GIY8,
UniProt Related Accession:P13747
Molecular Weight:40,157 Da
NCBI Full Name:HLA class I histocompatibility antigen, alpha chain E
NCBI Synonym Full Names:major histocompatibility complex, class I, E
NCBI Official Symbol:HLA-E  
NCBI Official Synonym Symbols:MHC; QA1; EA1.2; EA2.1; HLA-6.2  
NCBI Protein Information:HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC class I antigen E; lymphocyte antigen
UniProt Protein Name:HLA class I histocompatibility antigen, alpha chain E
UniProt Synonym Protein Names:MHC class I antigen E
UniProt Gene Name:HLA-E  
UniProt Entry Name:HLAE_HUMAN
Anti-HLA-E Antibody (CAB14206)Western blot analysis of extracts of various cell lines, using HLA-E antibody (CAB14206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

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