Anti-HMGB2 Antibody (CAB9168)

SKU:
CAB9168
€129 - €349

Description

Technical Manual

Anti-HMGB2 Antibody

Product Name:Anti-HMGB2 Antibody
Product SKU:CAB9168
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008]

Immunogen:A synthesized peptide derived from human HMGB2
Sequence:

Email for sequence

Gene ID:3148
Swiss Prot:P26583
Synonyms:HMG2
Calculated MW:24kDa
Observed MW:24KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence
Positive Samples:HeLa, HepG2, RAW264.7, THP-1, Mouse testis, Rat testis, Rat spleen, Rat thymus
Cellular Location:

Product Images

Anti-HMGB2 Antibody (CAB9168)

Western blot - HMGB2 Rabbit mAb (CAB9168)

Uniprot Information

UniProt Protein Function:HMGB2: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Belongs to the HMGB family.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; extracellular space; protein complex; perinuclear region of cytoplasm; cytoplasm; condensed chromosome; nucleus

Molecular Function:protein domain specific binding; protein binding; RAGE receptor binding; DNA binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; transcription factor activity; DNA bending activity; single-stranded DNA binding; chemoattractant activity

Biological Process: positive regulation of nuclease activity; establishment and/or maintenance of chromatin architecture; DNA topological change; V(D)J recombination; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; male gonad development; spermatid nuclear differentiation; base-excision repair, DNA ligation; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nucleosome assembly; positive chemotaxis; response to steroid hormone stimulus; DNA ligation during DNA repair; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of megakaryocyte differentiation; DNA fragmentation during apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of DNA binding; cell structure disassembly during apoptosis

NCBI Summary:This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008]
UniProt Code:P26583
NCBI GenInfo Identifier:123374
NCBI Gene ID:3148
NCBI Accession:P26583.2
UniProt Secondary Accession:P26583,Q5U072, B2R4K8, D3DP37,
UniProt Related Accession:P26583
Molecular Weight:209
NCBI Full Name:High mobility group protein B2
NCBI Synonym Full Names:high mobility group box 2
NCBI Official Symbol:HMGB2
NCBI Official Synonym Symbols:HMG2
NCBI Protein Information:high mobility group protein B2; HMG-2; high-mobility group box 2; high mobility group protein 2; high-mobility group (nonhistone chromosomal) protein 2
UniProt Protein Name:High mobility group protein B2
UniProt Synonym Protein Names:High mobility group protein 2; HMG-2
Protein Family:High mobility group protein
UniProt Gene Name:HMGB2
UniProt Entry Name:HMGB2_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
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