The HMGCS2 Antibody (CAB14244) is a high-quality antibody developed for reliable detection and analysis of target proteins. Mevalonate is a key intermediate in the biosynthesis of cholesterol, coenzyme Q, and other important molecules. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. With its ability to bind to the HMGCS2 protein, researchers can accurately detect and analyze this enzyme in a variety of cell types.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HMGCS2 Antibody
SKU:
CAB14244
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HMGCS2
Positive Sample:
COLO 205, U-251 MG
Cellular Localization:
Mitochondrion.
Calculated MW:
57kDa
Observed MW:
50kDa
The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3158
RRID
AB_2761104
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HMGCS2 Rabbit pAb (CAB14244) at 1:7000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using HMGCS2 Rabbit pAb (CAB14244) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat liver using HMGCS2 Rabbit pAb (CAB14244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded human liver using HMGCS2 Rabbit pAb (CAB14244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
