Anti-HNF4A Antibody (CAB2085)

Antibody Name:	Anti-HNF4A Antibody
Antibody SKU:	CAB2085
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human HNF4A (NP_000448.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, 293T, SW620, Mouse liver, Mouse kidney, Mouse pancreas, Rat liver, Rat kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200-300 of human HNF4A (NP_000448.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EWAK YIPA FCEL PLDD QVAL LRAH AGEH LLLG ATKR SMVF KDVL LLGN DYIV PRHC PELA EMSR VSIR ILDE LVLP FQEL QIDD NEYA YLKA IIFF DPDA K
Gene ID:	3172
Uniprot:	P41235
Cellular Location:	Nucleus
Calculated MW:	43-56kDa
Observed MW:	52kDa

Synonyms:

HNF4A, FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14

Background:

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.

UniProt Protein Function:	HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Nuclear receptor; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; xenobiotic metabolic process; regulation of gastrulation; response to glucose stimulus; ornithine metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent
NCBI Summary:	The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
UniProt Code:	P41235
NCBI GenInfo Identifier:	148886624
NCBI Gene ID:	3172
NCBI Accession:	P41235.3
UniProt Secondary Accession:	P41235,O00659, O00723, Q14540, Q5QPB8, Q6B4V5, Q6B4V6 Q6B4V7, Q92653, Q92654, A5JW41, B2RPP8,
UniProt Related Accession:	P41235
Molecular Weight:
NCBI Full Name:	Hepatocyte nuclear factor 4-alpha
NCBI Synonym Full Names:	hepatocyte nuclear factor 4, alpha
NCBI Official Symbol:	HNF4A
NCBI Official Synonym Symbols:	TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
NCBI Protein Information:	hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1
UniProt Protein Name:	Hepatocyte nuclear factor 4-alpha
UniProt Synonym Protein Names:	Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4
Protein Family:	Hepatocyte nuclear factor
UniProt Gene Name:	HNF4A
UniProt Entry Name:	HNF4A_HUMAN

	Western blot analysis of extracts of various cell lines, using HNF4A antibody (CAB2085) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse liver using HNF4A Antibody (CAB2085) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat pancreas using HNF4A Antibody (CAB2085) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-HNF4A Antibody (CAB13998)	HNF4alpha/gamma Transcription Factor Activity Assay
Anti-Phospho-HNF4A-S304 Antibody (CABP0362)	HNF4alpha Colorimetric Cell-Based ELISA Kit
Anti-Phospho-HNF4A-S304 pAb Antibody (CABP0834)	HNF4alpha (Phospho-Ser313) Colorimetric Cell-Based ELISA Kit
Phospho-HNF4A (S313) Antibody (PACO00327)
HNF4A Antibody (PACO00919)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)