Anti-HNRNPL Antibody (CAB8430)

Antibody Name:	Anti-HNRNPL Antibody
Antibody SKU:	CAB8430
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 282-589 of human HNRNPL (NP_001524.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, HepG2, A-549, HT-29, Mouse ovary, Rat lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 282-589 of human HNRNPL (NP_001524.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TWDY TNPN LSGQ GDPG SNPN KRQR QPPL LGDH PAEY GGPH GGYH SHYH DEGY GPPP PHYE GRRM GPPV GGHR RGPS RYGP QYGH PPPP PPPP EYGP HADS PVLM VYGL DQSK MNCD RVFN VFCL YGNV EKVK FMKS KPGA AMVE MADG YAVD RAIT HLNN NFMF GQKL NVCV SKQP AIMP GQSY GLED GSCS YKDF SESR NNRF STPE QAAK NRIQ HPSN VLHF FNAP LEVT EENF FEIC DELG VKRP SSVK VFSG KSER SSSG LLEW ESKS DALE TLGF LNHY QMKN PNGP YPYT LKLC FSTA QHAS
Gene ID:	3191
Uniprot:	P14866
Cellular Location:	Cytoplasm, Nucleus, nucleoplasm
Calculated MW:	50kDa/64kDa
Observed MW:	64kDa

Synonyms:

HNRNPL, HNRPL, P/OKcl.14, hnRNP-L

Background:

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	hnRNP L: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: membrane; nucleoplasm; nucleus; ribonucleoprotein complex Molecular Function:protein binding; RNA binding Biological Process: gene expression; nuclear mRNA splicing, via spliceosome; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA processing
NCBI Summary:	Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P14866
NCBI GenInfo Identifier:	215274006
NCBI Gene ID:	3191
NCBI Accession:	P14866.2
UniProt Secondary Accession:	P14866,Q9H3P3, A6ND69, A6NIT8,
UniProt Related Accession:	P14866
Molecular Weight:	50,561 Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein L
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein L
NCBI Official Symbol:	HNRNPL
NCBI Official Synonym Symbols:	HNRPL; hnRNP-L; P/OKcl.14
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein L
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein L
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPL
UniProt Entry Name:	HNRPL_HUMAN

	Western blot analysis of extracts of various cell lines, using HNRNPL antibody (CAB8430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human stomach using HNRNPL antibody (CAB8430) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using HNRNPL antibody (CAB8430) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of 293T cells using HNRNPL antibody (CAB8430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-HNRNPL Antibody (CAB12447)

HNRNPL Antibody (PACO06558)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)