The Horse IgA (Immunoglobulin A) ELISA Kit from Assay Genie is a powerful tool for the precise measurement of IgA levels in equine serum, plasma, and other biological samples. This ELISA kit is designed with superior sensitivity and specificity to deliver accurate and reproducible results, making it perfect for a diverse array of research applications in the field of equine immunology. Immunoglobulin A, or IgA, is a key component of the horse's immune system, playing a crucial role in mucosal immunity and defending against pathogens. Monitoring IgA levels can provide valuable insights into the health and immune status of horses, making this ELISA kit essential for equine health studies, veterinary research, and diagnostic applications.
With the Horse IgA ELISA Kit from Assay Genie, researchers can delve deeper into the intricate mechanisms of equine immunity, paving the way for advancements in equine health and disease management. Trust in the accuracy and reliability of this ELISA kit to drive your research forward and unlock new discoveries in equine immunology.
Product Name:
Horse IgA ((Immunoglobulin A) ELISA Kit
SKU:
AEFI00076
Reactivity:
Horse
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.094 ng/mL
Range:
0.156-10 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
95-104%
86-101%
81-99%
EDTA Plasma (n = 5)
85-103%
84-101%
85-97%
Heparin Plasma (n = 5)
89-105%
87-101%
80-95%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-105
95
EDTA Plasma (n = 5)
85-105
96
Heparin Plasma (n = 5)
87-101
93
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
