The Mouse IgA (Immunoglobulin A) ELISA Kit is specifically designed for the quantitative detection of IgA levels in mouse serum, plasma, and other biological fluids. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Immunoglobulin A is an important component of the immune system, providing mucosal immunity and protection against pathogens. Elevated levels of IgA can indicate infections, autoimmune diseases, and inflammatory conditions, making it a valuable biomarker for monitoring immune response and disease progression. With this ELISA kit, researchers can easily measure IgA levels in mouse samples, providing valuable insights into the immune system function and disease mechanisms. Whether studying autoimmune disorders, infectious diseases, or inflammatory conditions, the Mouse IgA ELISA Kit is an essential tool for advancing research in immunology and related fields.
Product Name:
Mouse IgA (Immunoglobulin A) ELISA Kit
SKU:
MOES01187
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
