Description
HPV16 E2 Recombinant Protein (N-His)
HPV16 E2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Ser201, with an N-terminal His tag. Predicted molecular weight: 25.66 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Regulatory protein E2 is a ~41 kDa protein. Plays a role in the initiation of viral DNA replication. For research use only.
A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box.
| Product Name: | HPV16 E2 Recombinant Protein (N-His) |
| SKU: | GXRP09944 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | HPV16 |
| Synonyms: | Regulatory protein E2, E2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Ser201 |
| Calculated MW: | 25.66 kDa |
| UniProt: | P03120 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
