Description
HPV16 L2 Recombinant Protein (N-His)
HPV16 L2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg2-Ala473(Glu43Asp), with an N-terminal His tag. Predicted molecular weight: 53.11 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Minor capsid protein L2 is a ~50 kDa protein. Minor protein of the capsid that localizes along the inner surface of the virion, within the central cavities beneath the L1 pentamers. For research use only.
Plays a role in capsid stabilization through interaction with the major capsid protein L1. Once the virion enters the host cell, L2 escorts the genomic DNA into the nucleus by promoting escape from the endosomal compartments and traffic through the host Golgi network. Mechanistically, the C-terminus of L2 possesses a cell-penetrating peptide that protudes from the host endosome, interacts with host cytoplasmic retromer cargo and thereby mediates the capsid delivery to the host trans-Golgi network. Plays a role through its interaction with host dynein in the intracellular microtubule-dependent transport of viral capsid toward the nucleus.
| Product Name: | HPV16 L2 Recombinant Protein (N-His) |
| SKU: | GXRP10136 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | HPV16 |
| Synonyms: | Minor capsid protein L2, L2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg2-Ala473(Glu43Asp) |
| Calculated MW: | 53.11 kDa |
| UniProt: | P03107 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
