HRP Detection Reagent (Fluorometric) (BA0173)

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SKU:
BA0173
€379

Description

ELISA Kit Technical ManualMSDS

HRP Detection Reagent:

Assay Genie's fluorometric HRP detection reagent is a stable 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red TM) liquid substrate reagent formulated for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent product (Ex/Em = 530/590nm) when reacted with H2O2 by a peroxidase. The fluorescent product can also be visualized under a light source (530 ± 20 nm).

Applications:

Stabilized 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red TM) liquid substrate reagent for detecting horseradish peroxidase HRP activity, e.g. in ELISA. This reagent produces a highly fluorescent (530/590nm), pink-colored product upon reaction with a peroxidase. The fluorescent product can also be visualized under a light source (510-550 nm).

HRP Detection Reagent - Key Features

  • For both absorbance or fluorescence detection.
  • Highly sensitive.
  • Convenient and fast.

HRP Detection Reagent Information:

Kit Includes  HRP Detection Reagent 25 mL Reagent 100 mL 3% Stabilized H2O2
Method of Detection OD570nm, FL530/585nm
Samples Biological
Species All
Protocol Length 30 min
Size 500 tests
Storage Long-term storage, keep reagent at -20°C
Shelf Life 12 months

*Ampliflu Red is a trademark of Sigma-Aldrich

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Additional Information

Product type:
Assay
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