Hsc70 Rabbit Monoclonal Antibody

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SKU:
CAB0415
€129 - €349

Description

Technical Manual

Hsc70 Rabbit Monoclonal Antibody

Product Name:Hsc70 Rabbit Monoclonal Antibody
Product SKU:CAB0415
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Immunogen:A synthesized peptide derived from human Hsc70
Sequence:

Email for sequence

Gene ID:3312
Swiss Prot:P11142
Synonyms:HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71
Calculated MW:70kDa
Observed MW:71KDa
Reactivity:Human, Mouse, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting 
Positive Samples:HeLa, 293T, NIH/3T3, U-87MG, Mouse brain, Rat brain
Cellular Location:

Product Images

Hsc70 Rabbit Monoclonal Antibody

Western blot - Hsc70 Rabbit mAb (CAB0415)

Uniprot Information

UniProt Protein Function:HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; Heat shock protein; Chaperone; Nucleolus

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: nucleoplasm; spliceosome; extracellular space; focal adhesion; membrane; melanosome; plasma membrane; nucleolus; intracellular; ribonucleoprotein complex; nucleus; cytosol; ubiquitin ligase complex

Molecular Function:protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; ATPase activity; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: axon guidance; transcription, DNA-dependent; viral reproduction; protein folding; chaperone cofactor-dependent protein folding; regulation of cell cycle; neurotransmitter secretion; RNA splicing; response to unfolded protein; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; synaptic transmission; protein refolding; gene expression; post-Golgi vesicle-mediated transport; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:P11142
NCBI GenInfo Identifier:123648
NCBI Gene ID:3312
NCBI Accession:P11142.1
UniProt Secondary Accession:P11142,Q9H3R6,
UniProt Related Accession:P11142
Molecular Weight:646
NCBI Full Name:Heat shock cognate 71 kDa protein
NCBI Synonym Full Names:heat shock 70kDa protein 8
NCBI Official Symbol:HSPA8 
NCBI Official Synonym Symbols:LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p 
NCBI Protein Information:heat shock cognate 71 kDa protein; LPS-associated protein 1; heat shock 70kd protein 10; epididymis luminal protein 33; heat shock cognate protein 54; constitutive heat shock protein 70; lipopolysaccharide-associated protein 1; N-myristoyltransferase inhibitor protein 71; epididymis secretory sperm binding protein Li 72p
UniProt Protein Name:Heat shock cognate 71 kDa protein
UniProt Synonym Protein Names:Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1
UniProt Gene Name:HSPA8 
UniProt Entry Name:HSP7C_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
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