Anti-HSD17B8 Antibody (CAB12947)

Antibody Name:	Anti-HSD17B8 Antibody
Antibody SKU:	CAB12947
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B8 (NP_055049.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, BxPC-3, U-87MG, HeLa, Mouse liver, Mouse pancreas, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B8 (NP_055049.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASQ LQNR LRSA LALV TGAG SGIG RAVS VRLA GEGA TVAA CDLD RAAA QETV RLLG GPGS KEGP PRGN HAAF QADV SEAR AARC LLEQ VQAC FSRP PSVV VSCA GITQ DEFL LHMS EDDW DKVI AVNL KGTF LVTQ AAAQ ALVS NGCR GSII NISS IVGK VGNV GQTN YAAS KAGV IGLT QTAA RELG RHGI RCNS VLPG FIAT PMTQ KVPQ KVVD KITE MIPM GHLG DPED VADV VAFL ASED SGYI TGTS VEVT GGLF M
Gene ID:	7923
Uniprot:	Q92506
Cellular Location:	Mitochondrion matrix
Calculated MW:	26kDa
Observed MW:	27kDa

Synonyms:

HSD17B8, D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9

Background:

In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.

UniProt Protein Function:	HSD17B8: NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone. The heteroteramer with CBR4 has NADH-dependent 3- ketoacyl-acyl carrier protein reductase activity. May play a role in biosynthesis of fatty acids in mitochondria. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:	Protein type:Lipid Metabolism - androgen and estrogen; EC 1.1.1.239; Mitochondrial; EC 1.1.1.62; Oxidoreductase Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: mitochondrial matrix Molecular Function:3-hydroxyacyl-CoA dehydrogenase activity; estradiol 17-beta-dehydrogenase activity; protein binding Biological Process: estrogen biosynthetic process
NCBI Summary:	In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92506
NCBI GenInfo Identifier:	12643402
NCBI Gene ID:	7923
NCBI Accession:	Q92506.2
UniProt Secondary Accession:	Q92506,Q5STP7, Q9UIQ1, A6NLX7,
UniProt Related Accession:	Q92506
Molecular Weight:	26,974 Da
NCBI Full Name:	Estradiol 17-beta-dehydrogenase 8
NCBI Synonym Full Names:	hydroxysteroid 17-beta dehydrogenase 8
NCBI Official Symbol:	HSD17B8
NCBI Official Synonym Symbols:	KE6; FABG; HKE6; FABGL; RING2; H2-KE6; SDR30C1; D6S2245E; dJ1033B10.9
NCBI Protein Information:	estradiol 17-beta-dehydrogenase 8
UniProt Protein Name:	Estradiol 17-beta-dehydrogenase 8
UniProt Synonym Protein Names:	17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.-); Protein Ke6; Ke-6; Really interesting new gene 2 protein; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8 (EC:1.1.1.239)
Protein Family:	Estradiol 17 beta-dehydrogenase
UniProt Gene Name:	HSD17B8
UniProt Entry Name:	DHB8_HUMAN

	Western blot analysis of extracts of various cell lines, using HSD17B8 Antibody (CAB12947) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U-2 OS cells using HSD17B8 Polyclonal Antibody (CAB12947) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

HSD17B8 Antibody (PACO09812)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)