Anti-HSF2 Antibody (CAB2747)

Antibody Name:	Anti-HSF2 Antibody
Antibody SKU:	CAB2747
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human HSF2

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	293T, HepG2, SW620, BT-474

Immunogen:	Recombinant protein of human HSF2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3298
Uniprot:	Q03933
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	58kDa/60kDa
Observed MW:	60kDa

Synonyms:	HSF2, HSF 2, HSTF 2, HSTF2
Background:	The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6q22.31 Cellular Component: cytoplasm; nucleus Molecular Function:protein homodimerization activity; transcription coactivator activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis
NCBI Summary:	The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
UniProt Code:	Q03933
NCBI GenInfo Identifier:	462334
NCBI Gene ID:	3298
NCBI Accession:	Q03933.1
UniProt Secondary Accession:	Q03933,Q0VAH9, Q2M1K4, Q9H445, B4DGJ4,
UniProt Related Accession:	Q03933
Molecular Weight:	58,293 Da
NCBI Full Name:	Heat shock factor protein 2
NCBI Synonym Full Names:	heat shock transcription factor 2
NCBI Official Symbol:	HSF2
NCBI Official Synonym Symbols:	HSF 2; HSTF 2
NCBI Protein Information:	heat shock factor protein 2
UniProt Protein Name:	Heat shock factor protein 2
UniProt Synonym Protein Names:	Heat shock transcription factor 2; HSTF 2
Protein Family:	Heat shock factor protein
UniProt Gene Name:	HSF2
UniProt Entry Name:	HSF2_HUMAN

	Western blot analysis of extracts of various cell lines, using HSF2 antibody (CAB2747) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat liver using HSF2 antibody (CAB2747) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A549 cells using HSF2 antibody (CAB2747). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-HSF2 Antibody (CAB12264)	Human HSF2 ELISA Kit
HSF2 Antibody (PACO04082)	Human HSF2 (Heat Shock Transcription Factor 2) CLIA Kit (HUES01032)
HSF2 Antibody (PACO09818)	HSF2 Colorimetric Cell-Based ELISA

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)