The HSPA4L Antibody (CAB17637) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific for human samples and has been validated for use in Western blot applications.HSPA4L, also known as heat shock 70 kDa protein 4-like, plays a crucial role in maintaining protein homeostasis under stress conditions. Its function in protein folding and quality control makes it essential for cellular survival and function. Research into HSPA4L can provide insights into mechanisms of cell survival and potential therapeutic targets for diseases related to protein misfolding.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HSPA4L Antibody
SKU:
CAB17637
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APG1, APG-1, HSPH3, Osp94, HSPA4L
Positive Sample:
U-87MG, K-562, HeLa, Mouse brain, Rat testis, Rat brain
Cellular Localization:
Cytoplasm, Cytosol, Nucleus.
Calculated MW:
95kDa
Observed MW:
115kDa
The protein encoded by this gene is heat shock inducible and may act as a chaperone. The encoded protein can protect the heat-shocked cell against the harmful effects of aggregated proteins. This gene is highly expressed in leukemia cells and may be a good target for therapeutic intervention. Several transcripts encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
22824
RRID
AB_2769890
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HSPA4L Rabbit pAb (CAB17637) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of A431 cells using HSPA4L Rabbit pAb (CAB17637) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
