Human 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) ELISA Kit
The Human 17-beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17B14) ELISA Kit is a highly reliable and sensitive assay designed for the precise detection of HSD17B14 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides outstanding specificity, ensuring accurate and reproducible results for a wide range of research applications.HSD17B14 is an important enzyme involved in the metabolism of steroid hormones and has been linked to various diseases, including cancer and hormone-related disorders.
Studying HSD17B14 levels can provide valuable insights into these conditions and may aid in the development of potential therapeutic strategies.With its advanced technology and precise detection capabilities, the Human HSD17B14 ELISA Kit is an essential tool for researchers looking to explore the role of HSD17B14 in health and disease. Trust in this kit to deliver reliable results and advance your scientific investigations.
Product Name:
Human 17-Beta-Hydroxysteroid Dehydrogenase Type 14 (HSD17b14) ELISA Kit
Product Code:
HUDL01373
Size:
96 Assays
Target:
HSD17b14
Synonyms:
DHRS10, RetSDR3, SDR47C1, Retinal Short-Chain Dehydrogenase/Reductase 3, Short Chain Dehydrogenase/Reductase Family 47C,Member 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
