Human 5-hydroxytryptamine Receptor 7 (HTR7) ELISA Kit
The Human 5-Hydroxytryptamine Receptor 7 (HTR7) ELISA Kit is a highly reliable and accurate tool for the measurement of 5-HT7 receptor levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.The 5-HT7 receptor is a key player in the serotonin system, influencing a wide range of physiological and psychological processes. Dysregulation of this receptor has been linked to various neurological and psychiatric disorders, making it a crucial target for therapeutic interventions and drug development.
By using the Human 5-Hydroxytryptamine Receptor 7 ELISA Kit, researchers can gain valuable insights into the role of the 5-HT7 receptor in health and disease, paving the way for novel treatment strategies and personalized medicine approaches. Trust in this innovative ELISA kit to advance your research and accelerate scientific discovery in the field of neuroscience.
Product Name:
Human 5-hydroxytryptamine Receptor 7 (HTR7) ELISA Kit
Product Code:
HUDL01408
Size:
96 Assays
Target:
HTR7
Synonyms:
5-HT-X, Serotonin receptor 7
Detection Method:
Sandwich
Range:
78-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
