Description
Human ABCA4 Recombinant Protein (N-His)
Human ABCA4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly1398-Asn1727, with an N-terminal His tag. Predicted molecular weight: 39.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Retinal-specific phospholipid-transporting ATPase ABCA4 is a ~255 kDa protein. Flippase that catalyzes in an ATP-dependent manner the transport of retinal-phosphatidylethanolamine conjugates like 11-cis and all-trans isomers of N-retinylidene-phosphatidylethanolamine (N-Ret-PE) from the lumen to the cytoplasmic leaflet of photoreceptor outer segment disk membranes, where 11-cis-retinylidene-phosphatidylethanolamine is then isomerized to its all-trans isomer and reduced by RDH8 to produce all-trans-retinol. For research use only.
This transport activity ensures that all-trans-retinal generated from photoexcitation and 11-cis-retinal not needed for the regeneration of rhodopsin and cone opsins are effectively cleared from the photoreceptors, therefore preventing their accumulation and the formation of toxic bisretinoid. Displays ATPase activity in vitro in absence of retinal substrate. May display GTPase activity that is strongly influenced by the lipid environment and the presence of retinoid compounds. Binds the unprotonated form of N-retinylidene-phosphatidylethanolamine with high affinity in the absence of ATP, and ATP binding and hydrolysis induce a protein conformational change that causes N-retinylidene-phosphatidylethanolamine release.
| Product Name: | Human ABCA4 Recombinant Protein (N-His) |
| SKU: | GXRP01941 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RIM protein, Stargardt disease protein, Retinal-specific ATP-binding cassette transporter, RIM ABC transporter, ATP-binding cassette sub-family A member 4, ABCA4, RmP, ABCR, Retinal-specific phospholipid-transporting ATPase ABCA4 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly1398-Asn1727 |
| Calculated MW: | 39.16 kDa |
| UniProt: | P78363 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
