Description
Human ACAT1 Recombinant Protein (N-His)
Human ACAT1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala111-Leu427, with an N-terminal His tag. Predicted molecular weight: 35.82 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Acetyl-CoA acetyltransferase, mitochondrial (ACAT1) is a ~45 kDa protein. This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. For research use only.
Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
| Product Name: | Human ACAT1 Recombinant Protein (N-His) |
| SKU: | GXRP01277 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ACAT, Acetoacetyl-CoA thiolase, MAT, Acetyl-CoA acetyltransferase, mitochondrial, ACAT1, T2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala111-Leu427 |
| Calculated MW: | 35.82 kDa |
| UniProt: | P24752 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |