The Human AChR (Anti-Acetylcholine Receptor) ELISA Kit is specifically designed for the accurate measurement of anti-acetylcholine receptor antibodies in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications. Anti-acetylcholine receptor antibodies play a critical role in autoimmune diseases such as myasthenia gravis, where they target the acetylcholine receptors at the neuromuscular junction, leading to muscle weakness and fatigue.
By detecting these antibodies, researchers can better understand the underlying mechanisms of autoimmune diseases and potentially develop targeted therapies for patients. With its easy-to-use protocol and high-quality components, the Human AChR ELISA Kit is a valuable tool for researchers studying autoimmune diseases, neurology, and immunology. It provides a comprehensive solution for accurately measuring anti-acetylcholine receptor antibodies and advancing our understanding of these complex conditions.
Product Name:
Human AChR Autoantibodies ELISA Kit
SKU:
HUFI02215
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
82-100%
84-95%
92-103%
EDTA Plasma (n = 5)
83-100%
84-92%
88-96%
Heparin Plasma (n = 5)
82-100%
80-95%
89-101%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-98
90
EDTA Plasma (n = 5)
85-98
95
Heparin Plasma (n = 5)
86-99
94
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
