SynonymsACVRL1,ACVRLK1,HHT2,ORW2,SKR3,TSR-I,TGF-B superfamily receptor type I,Activin A Receptor Type II-Like 1,Serine/Threonine-Protein Kinase Receptor R3
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL00142
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P37023
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ACVRL1,ACVRLK1,HHT2,ORW2,SKR3,TSR-I,TGF-B superfamily receptor type I,Activin A Receptor Type II-Like 1,Serine/Threonine-Protein Kinase Receptor R3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Activin Receptor Like Kinase 1 (ALK1) ELISA Kit
The Human Activin Receptor-Like Kinase 1 (ALK1) ELISA Kit is a valuable tool for accurately measuring levels of ALK1 in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ALK1 is a key receptor involved in angiogenesis and vascular development, playing a critical role in various diseases such as hereditary hemorrhagic telangiectasia (HHT) and cancer.
Understanding the expression and activity of ALK1 is essential for studying these conditions and exploring potential therapeutic interventions.With the Human ALK1 ELISA Kit, researchers can obtain precise and quantitative data on ALK1 levels, making it a valuable asset for advancing research in the fields of oncology, cardiovascular biology, and vascular biology. Trust in this kit to deliver accurate results and drive impactful discoveries in your research endeavors.
Product Name:
Human Activin Receptor Like Kinase 1 (ALK1) ELISA Kit
Product Code:
HUDL00142
Size:
96 Assays
Target:
ALK1
Synonyms:
ACVRL1, ACVRLK1, HHT2, ORW2, SKR3, TSR-I, TGF-B superfamily receptor type I, Activin A Receptor Type II-Like 1, Serine/Threonine-Protein Kinase Receptor R3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
