Human Adrenocorticotropic Hormone (ACTH) ELISA Kit
The Human Adrenocorticotropic Hormone (ACTH) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ACTH levels in human serum, plasma, and cell culture supernatants. ACTH is a key hormone involved in regulating the body's response to stress, controlling cortisol production, and influencing various biological processes. Abnormal levels of ACTH have been associated with conditions such as Cushing's syndrome, Addison's disease, and pituitary gland disorders.
This ELISA kit offers reliable and reproducible results, making it an essential tool for researchers studying endocrine disorders, neuroendocrinology, and stress response mechanisms. With its high sensitivity and specificity, this kit is suitable for a wide range of research applications and has the potential to aid in the development of novel diagnostic and therapeutic strategies for ACTH-related conditions.
Product Name:
Human Adrenocorticotropic Hormone (ACTH) ELISA Kit
Product Code:
HUDL00048
Size:
96 Assays
Target:
ACTH
Synonyms:
Corticotropin, Adrenocorticotropin
Detection Method:
Competition
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
