Human ACTH (Adrenocorticotropic Hormone) ELISA Kit
The Human ACTH (Adrenocorticotropic Hormone) ELISA Kit is a high-quality assay designed for the precise detection of ACTH levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ACTH is a key hormone involved in the regulation of the body's stress response and plays a crucial role in the synthesis and release of cortisol from the adrenal glands. Dysregulation of ACTH levels can contribute to conditions such as Cushing's syndrome and other adrenal disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its advanced technology and ease of use, the Human ACTH ELISA Kit from Assay Genie is an essential tool for researchers investigating the intricate mechanisms of hormone regulation and looking to advance our understanding of various endocrine disorders.
Product Name:
Human ACTH (Adrenocorticotropic Hormone) ELISA Kit
SKU:
HUES01414
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
