Description
Human ANKRD27 Recombinant Protein (N-His)
Human ANKRD27 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met654-Ser902, with an N-terminal His tag. Predicted molecular weight: 28.99 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ankyrin repeat domain-containing protein 27 (ANKRD27) is a ~116 kDa protein. May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics. For research use only.
May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation. Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7. Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38. Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1.
| Product Name: | Human ANKRD27 Recombinant Protein (N-His) |
| SKU: | GXRP05760 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ANKRD27, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met654-Ser902 |
| Calculated MW: | 28.99 kDa |
| UniProt: | Q96NW4 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
