Description
Human APAF1 Recombinant Protein (N-His)
Human APAF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser31-Phe350, with an N-terminal His tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Apoptotic protease-activating factor 1 (APAF1) is a ~141 kDa protein. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. For research use only.
This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
| Product Name: | Human APAF1 Recombinant Protein (N-His) |
| SKU: | GXRP00079 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | APAF1, APAF-1, Apoptotic protease-activating factor 1, KIAA0413 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser31-Phe350 |
| UniProt: | O14727 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |