Human APP (Amyloid-beta precursor protein) ELISA Kit
The Human APP (Amyloid Beta Precursor Protein) ELISA Kit is a powerful tool for detecting and quantifying levels of amyloid beta precursor protein in human samples. This kit is highly sensitive and specific, offering reliable and reproducible results for research purposes. Amyloid beta precursor protein is a key player in the pathogenesis of Alzheimer's disease, as it is cleaved to produce amyloid beta peptides that accumulate as plaques in the brain. By measuring levels of this protein, researchers can gain insights into the progression of Alzheimer's disease and potentially identify new therapeutic targets.
This ELISA kit is easy to use and provides fast results, making it an ideal choice for studying neurodegenerative disorders and investigating potential treatments. Whether you are studying Alzheimer's disease, dementia, or other neurological conditions, the Human APP ELISA Kit is an invaluable tool for advancing your research.
Product Name:
Human APP (Amyloid-beta precursor protein) ELISA Kit
SKU:
HUFI03423
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.563 ng/mL
Range:
0.938-60 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
85-104%
86-99%
89-105%
EDTA Plasma (n = 5)
82-97%
82-99%
84-101%
Heparin Plasma (n = 5)
80-99%
87-100%
83-100%
Recovery:
Sample
1
2
Serum (n = 5)
87-101
94
EDTA Plasma (n = 5)
88-105
97
Heparin Plasma (n = 5)
97-103
99
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
