The Human APP (Amyloid Precursor Protein) CLIA Kit is a cutting-edge assay designed for the accurate measurement of amyloid precursor protein levels in human samples including serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and specificity, guaranteeing precise and reproducible results that are essential for various research applications.Amyloid precursor protein (APP) is a key player in the pathogenesis of various diseases, particularly Alzheimer's disease.
By measuring APP levels, researchers can gain valuable insights into the mechanisms underlying neurodegenerative disorders and potentially identify new therapeutic targets. With its superior performance and broad applicability, the Human APP CLIA Kit is an indispensable tool for investigating the role of APP in disease pathogenesis and drug development efforts.
Product Name:
Human APP (Amyloid Precursor Protein) CLIA Kit
SKU:
HUES00696
Target:
Human APP (Amyloid Precursor Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human APP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human APP. You can calculate the concentration of Human APP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
92-106
93-108
Average (%)
100
98
100
1:4
Range (%)
87-98
89-103
87-99
Average (%)
93
95
94
1:8
Range (%)
92-108
97-113
87-98
Average (%)
100
104
92
1:16
Range (%)
93-105
89-103
86-100
Average (%)
99
94
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
97
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.42
135.33
437.24
47.86
129.7
438.18
Standard deviation
5.78
11.26
42.41
5.07
11.18
40.4
C V (%)
11.94
8.32
9.7
10.59
8.62
9.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human APP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human APP in samples. No significant cross-reactivity or interference between Human APP and analogues was observed.
