Description
Human ASF1A Recombinant Protein (N-His)
Human ASF1A Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-His150, with an N-terminal His tag. Predicted molecular weight: 19.18 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Histone chaperone ASF1A is a ~22 kDa protein. Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. For research use only.
Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Promotes homologous recombination-mediated repair of double-strand breaks (DSBs) at stalled or collapsed replication forks: acts by mediating histone replacement at DSBs, leading to recruitment of the MMS22L-TONSL complex and subsequent loading of RAD51. Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' and acetylation at 'Lys-14' (H3K9me1K14ac) marks, and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
| Product Name: | Human ASF1A Recombinant Protein (N-His) |
| SKU: | GXRP04925 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | hAsf1, CCG1-interacting factor A, hAsf1a, Anti-silencing function protein 1 homolog A, Histone chaperone ASF1A, CIA, ASF1A, hCIA |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-His150 |
| Calculated MW: | 19.18 kDa |
| UniProt: | Q9Y294 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
