Description
Human ATF4 Recombinant Protein (N-His)
Human ATF4 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Pro351, with an N-terminal His tag. Predicted molecular weight: 40.75 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Cyclic AMP-dependent transcription factor ATF-4 (ATF4/CREB2) is a ~38 kDa protein. Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). For research use only.
Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell recovery. Promotes the transcription of genes linked to amino acid sufficiency and resistance to oxidative stress to protect cells against metabolic consequences of ER oxidation.
| Product Name: | Human ATF4 Recombinant Protein (N-His) |
| SKU: | GXRP03397 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | TaxREB67, ATF4, cAMP-dependent transcription factor ATF-4, TXREB, CREB2, cAMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67, Activating transcription factor 4, CREB-2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-responsive element-binding protein 2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Pro351 |
| Calculated MW: | 40.75 kDa |
| UniProt: | P18848 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
