Description
Human ATG5 Recombinant Protein (N-His)
Human ATG5 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Asp275, with an N-terminal His tag. Predicted molecular weight: 34.74 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Autophagy protein 5 (ATG5) is a ~32 kDa protein. Involved in autophagic vesicle formation. For research use only.
Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation.
| Product Name: | Human ATG5 Recombinant Protein (N-His) |
| SKU: | GXRP02848 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | APG5-like, Apoptosis-specific protein, ASP, APG5L, ATG5, Autophagy protein 5 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Asp275 |
| Calculated MW: | 34.74 kDa |
| UniProt: | Q9H1Y0 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
