Human ATP Binding Cassette Transporter C13 (ABCC13) ELISA Kit
The Human ATP-Binding Cassette Transporter C13 (ABCC13) ELISA Kit is a powerful tool for the quantitative detection of ABCC13 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable asset for a variety of research applications.ABCC13 is a member of the ATP-binding cassette (ABC) transporter family, playing a vital role in cellular transportation and drug resistance mechanisms. Its dysregulation has been associated with various diseases, including cancer and drug resistance, highlighting its importance as a potential therapeutic target.
This ELISA kit provides researchers with a reliable method for studying ABCC13 expression levels, enabling a deeper understanding of its impact on disease development and progression. With its user-friendly protocols and efficient performance, the Human ABCC13 ELISA Kit is a valuable tool for advancing scientific knowledge and potentially identifying new treatment strategies.
Product Name:
Human ATP Binding Cassette Transporter C13 (ABCC13) ELISA Kit
Product Code:
HUDL00021
Size:
96 Assays
Target:
ABCC13
Synonyms:
ABC-C13, PRED6, C21orf73, Putative ATP-binding cassette sub-family C member 13
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
