Human ATP Binding Cassette Transporter C2 (ABCC2) ELISA Kit
The Human ATP-Binding Cassette Transporter C2 (ABCC2) ELISA Kit is a cutting-edge tool for measuring ABCC2 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications. ABCC2, also known as the multidrug resistance-associated protein 2 (MRP2), is a vital transporter protein involved in drug metabolism and detoxification. Its dysfunction has been linked to drug resistance in cancer cells and various liver disorders.
The Human ABCC2 ELISA Kit enables researchers to quantify ABCC2 levels, providing valuable insights into drug resistance mechanisms and potential therapeutic targets. By utilizing the Human ABCC2 ELISA Kit, researchers can further understand the role of ABCC2 in drug transport and cellular detoxification processes, paving the way for innovative treatments and personalized medicine strategies tailored to individual patient needs.
Product Name:
Human ATP Binding Cassette Transporter C2 (ABCC2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
