SynonymsKIAP,LIVIN,ML-IAP,MLIAP,RNF50,Melanoma Inhibitor Of Apoptosis Protein,Kidney Inhibitor Of Apoptosis Protein,Livin Inhibitor-Of-Apoptosis,RING finger protein 50
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL00331
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q96CA5
Sensitivity:
0.073ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KIAP,LIVIN,ML-IAP,MLIAP,RNF50,Melanoma Inhibitor Of Apoptosis Protein,Kidney Inhibitor Of Apoptosis Protein,Livin Inhibitor-Of-Apoptosis,RING finger protein 50
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Baculoviral IAP Repeat Containing Protein 7 (BIRC7) ELISA Kit
The Human BIRC7 (Baculoviral IAP Repeat Containing Protein 7) ELISA Kit is specifically designed for the accurate quantification of BIRC7 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it suitable for a wide range of research applications.BIRC7 is a key protein that plays a crucial role in inhibiting apoptosis and promoting cell survival.
Dysregulation of BIRC7 has been associated with various diseases, including cancer, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human BIRC7 ELISA Kit provides researchers with a powerful tool to study the role of BIRC7 in disease development and progression, ultimately leading to a better understanding of potential therapeutic targets.
Product Name:
Human Baculoviral IAP Repeat Containing Protein 7 (BIRC7) ELISA Kit
Product Code:
HUDL00331
Size:
96 Assays
Target:
BIRC7
Synonyms:
KIAP, LIVIN, ML-IAP, MLIAP, RNF50, Melanoma Inhibitor Of Apoptosis Protein, Kidney Inhibitor Of Apoptosis Protein, Livin Inhibitor-Of-Apoptosis, RING finger protein 50
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
