Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
The Human bFGF (FGF2) CLIA Kit is a cutting-edge tool designed for the precise measurement of basic fibroblast growth factor (bFGF or FGF2) levels in human samples. This kit offers superior sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.bFGF (FGF2) is a key growth factor that plays a critical role in cell proliferation, tissue regeneration, and wound healing. Dysregulation of bFGF levels has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions. As such, this CLIA kit serves as a valuable resource for investigating the role of bFGF in disease pathogenesis and developing potential therapeutic interventions.With its advanced technology and reliable performance, the Human bFGF (FGF2) CLIA Kit is an indispensable tool for researchers seeking to enhance their understanding of bFGF biology and its involvement in health and disease.
Product Name:
Human bFGF/FGF2 (Basic Fibroblast Growth Factor) CLIA Kit
SKU:
HUES00347
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 pg/mL
Detection range:
12.5-800 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
