Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
The Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit is specifically designed for the quantitative assessment of bFGF/FGF2 levels in various human biological samples. Basic fibroblast growth factor, also known as FGF2, plays a fundamental role in cell growth, wound healing, and tissue regeneration processes. As a potent angiogenic factor, bFGF/FGF2 is involved in various cellular functions essential for development and tissue repair. Accurate measurement of bFGF/FGF2 levels is vital for understanding its impact on angiogenesis, cell proliferation, and tissue regeneration mechanisms. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. With stringent quality control measures in place, the kit guarantees robust performance and ease of use, making it an excellent choice for both research and clinical applications. Rely on the precision and reliability of Assay Genie's bFGF/FGF2 ELISA Kit for accurate quantification of this critical growth factor in your studies.
Product Name:
Human bFGF/FGF2 (Basic Fibroblast Growth Factor) ELISA Kit
SKU:
AEES00078
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
