Description
Human BRAT1 Recombinant Protein (N-His)
Human BRAT1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Gly291, with an N-terminal His tag. Predicted molecular weight: 33.38 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Integrator complex assembly factor BRAT1 is a ~88 kDa protein. Component of a multiprotein complex required for the assembly of the RNA endonuclease module of the integrator complex. For research use only.
Associates with INTS9 and INTS11 in the cytoplasm and blocks the active site of INTS11 to inhibit the endonuclease activity of INTS11 before formation of the full integrator complex. Following dissociation of WDR73 of the complex, BRAT1 facilitates the nuclear import of the INTS9-INTS11 heterodimer. In the nucleus, INTS4 is integrated to the INTS9-INTS11 heterodimer and BRAT1 is released from the mature RNA endonuclease module by inositol hexakisphosphate (InsP6). BRAT1 is also involved in DNA damage response; activates kinases ATM, SMC1A and PRKDC by modulating their phosphorylation status following ionizing radiation (IR) stress.
| Product Name: | Human BRAT1 Recombinant Protein (N-His) |
| SKU: | GXRP11626 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | BAAT1, BRAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1, C7orf27, Integrator complex assembly factor BRAT1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Gly291 |
| Calculated MW: | 33.38 kDa |
| UniProt: | Q6PJG6 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
