The Human Bridging Integrator 1 (BIN1) ELISA Kit is a powerful tool for researchers studying the BIN1 protein in human samples. This kit allows for the precise quantification of BIN1 levels in serum, plasma, and cell culture supernatants with high sensitivity and specificity.BIN1 is known to play a key role in various cellular processes, including membrane trafficking and synaptic vesicle recycling. Dysregulation of BIN1 has been implicated in diseases such as Alzheimer's and cancer, making it a valuable biomarker for understanding disease mechanisms and developing targeted therapies.
With its reliable and reproducible results, the Human BIN1 ELISA Kit is suitable for a wide range of research applications, providing researchers with the tools they need to advance their studies in the field of cellular biology and disease research.
Product Name:
Human Bridging Integrator 1 (BIN1) ELISA Kit
Product Code:
HUDL00328
Size:
96 Assays
Target:
BIN1
Synonyms:
AMPH2, AMPHL, SH3P9, Amphiphysin II, Myc Box-Dependent-Interacting Protein 1, Amphiphysin-like protein
Detection Method:
Sandwich
Range:
1.56-100pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
